购物车
- 全部删除
- 您的购物车当前为空
Rigosertib sodium (ON-01910) 是一种选择性的非 ATP 竞争性PLK1抑制剂,IC50值为 9 nM。它是一种多激酶抑制剂和选择性抗癌剂,通过抑制PI3K/Akt 途径诱导细胞凋亡,促进组蛋白 H2AX 的磷酸化并诱导细胞周期中的 G2/M 期停滞。
Rigosertib sodium (ON-01910) 是一种选择性的非 ATP 竞争性PLK1抑制剂,IC50值为 9 nM。它是一种多激酶抑制剂和选择性抗癌剂,通过抑制PI3K/Akt 途径诱导细胞凋亡,促进组蛋白 H2AX 的磷酸化并诱导细胞周期中的 G2/M 期停滞。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
1 mg | ¥ 169 | 现货 | |
5 mg | ¥ 347 | 现货 | |
10 mg | ¥ 598 | 现货 | |
25 mg | ¥ 1,180 | 现货 | |
50 mg | ¥ 2,190 | 现货 | |
100 mg | ¥ 3,730 | 现货 | |
200 mg | ¥ 4,960 | 现货 | |
1 mL x 10 mM (in DMSO) | ¥ 355 | 现货 |
产品描述 | Rigosertib sodium (ON-01910), a non-ATP-competitive inhibitor of PLK1(IC50=9 nM), exhibits 30-fold higher specificity activity over Plk2 and no effect on Plk3. |
靶点活性 | CDK1:260 nM (IC50), Fyn:182 nM (IC50), PDGFR:18 nM (IC50), FLK1:42 nM (IC50), PLK1:9 nM (IC50), PLK2:260 nM (IC50), BCR-ABL:32 nM (IC50), Src:155 nM (IC50) |
激酶实验 | In vitro enzyme assays for PLK1: Recombinant PLK1 (10 ng) is incubated with different concentrations of Rigosertib in a 15 μL reaction mixture (50 mM HEPES, 10 mM MgCl2, 1 mM EDTA, 2 mM Dithiothreitol, 0.01% NP-40 [pH 7.5]) for 30 min at room temperature. Kinase reactions are performed for 20 min at 30 °C in a volume of 20 μL (15 μL enzyme + inhibitor, 2 μL 1 mM ATP), 2 μL of γ32P-ATP (40 μCi), and 1 μL of recombinant Cdc25C (100 ng) or casein (1 μg) substrates. Reactions are terminated by boiling for 2 min in 20 μL of 2× Laemmli buffer. Phosphorylated substrates are separated by 18% SDS-PAGE. The gels are dried and exposed to X-ray film for 3-10 min. |
细胞实验 | Cells are grown in either DMEM or RPMI supplemented with 10% fetal bovine serum and 1 unit/mL penicillin-streptomycin solution. Tumor cells are plated into six-well dishes at a density of 1 × 105cells/mL/well, and Rigosertib is added 24 hours later at various concentrations. Cell counts are determined from duplicate wells after 96-hour of treatment. The total number of viable cells is determined by trypan blue exclusio(Only for Reference) |
别名 | Rigosertib, Estybon, ON-01910 |
分子量 | 473.47 |
分子式 | C21H24NNaO8S |
CAS No. | 592542-60-4 |
存储 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | |||||||||||||||||||||||||||||||||||
溶解度信息 | H2O: 87 mg/mL (183.7 mM) Ethanol: < 1 mg/mL (insoluble or slightly soluble) DMSO: 88 mg/mL (185.9 mM) | |||||||||||||||||||||||||||||||||||
溶液配制表 | ||||||||||||||||||||||||||||||||||||
H2O/DMSO
|
版权所有©2015-2024 TargetMol Chemicals Inc.保留所有权利.